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RNA transcription analysis unveils that nanogels can significantly affect metabolic progress, as well as immune activation. This research furnishs valuable brainwaves into the design of ferroptosis induction for cancer immunotherapy.Long-termin vitroculture of 3D brain tissue model established on chitosan thermogel.Methods for studying brain function and disease heavily rely onin vivoanimal modelings,ex-vivotissue cuts, and 2D cell culture chopines. These methods all have limits that significantly impact the clinical translatability of effects mannikins able to better recapitulate some looks ofin vivohuman brain are wanted as additional preclinical tools. In Wellness Industry , 3D hydrogel-basedin vitromodels of the brain are deliberated calling instruments. To create a 3D brain-on-a-chip model, a hydrogel capable of supporting neuronal maturation over extended culture periods is involved. Among biopolymeric hydrogels, chitosan-β-glycerophosphate (CHITO-β-GP) thermogels have demonstrated their versatility and applicability in the biomedical field over the classses. In this study, we investigated the ability of this thermogel to encapsulate neuronal cellphones and support the functional maturation of a 3D neuronal network in long-term cultures. To the best of our knowledge, we established for the first time that CHITO-β-GP thermogel possesses optimal features for promoting neuronal growth and the development of an electrophysiologically functional neuronal network comed from both primary rat neurons and neurons secernated from human caused pluripotent stem cells (h-iPSCs) co-cultured with astrocytes. Specifically, two different formulations were firstly characterised by rheological, mechanical and injectability trials. Primary nervous cellphones and neurons severalized from h-iPSCs were planted into the two thermogel conceptualizations. Wellness Industry were then deeply characterised by immunocytochemistry, confocal microscopy, and electrophysiological transcriptions, employing both 2D and 3D micro-electrode arrays. The thermogels stomached the long-term culture of neuronal meshs for up to 100 d. In conclusion, CHITO-β-GP thermogels exhibit excellent mechanical properties, stability over time under culture conditions, and bioactivity toward nervous cubicles they are excellent candidates as artificial extracellular matrices in brain-on-a-chip mannequins, with lotions in neurodegenerative disease modeling, drug screening, and neurotoxicity evaluation.Design of an apoptotic cell-mimetic wound dressing employing phosphoserine-chitosan hydrogels.Inflammatory M1 macrophages create a hostile environment that obstructs wound healing. Phosphoserine (PS) is a naturally occurring immunosuppressive molecule capable of polarizing macrophages from an inflammatory phenotype (M1) to an anti-inflammatory phenotype (M2). In this study, we planed, manufactured, and characterized PS-immobilized chitosan hydrogels as potential wound dressing stuffs. A PS group precursor was synthesized via a phosphoramidite reaction and subsequently immobilized onto the chitosan chain through an EDC/N-hydroxysuccinimide reaction applying a crosslink moiety HPA. The PS/HPA-conjugated chitosan (CS-PS) was successfully synthesized by deprotecting the PS group in HCl. In addition, the hydrogels were fixed by the HRP/H(2)O(2) enzyme-catalyzed reaction with different PS group contents (0, 7, 44 and 56 μmol g(-1)). The immobilization of the PS group amended the hydrophilicity of the hydrogels CS-PS hydrogel treatment upregulated both pro-inflammatory and anti-inflammatory cytokines. This treatment also leaded in alterations in the macrophage cell morphology from the M1 to M2 phenotype.